{
  "title": "Trita C-CAMP - selected internal experimental evidence",
  "prepared_on": "2026-10-19",
  "evidence_level": "Qualitative internal development records; not clinical validation",
  "image_handling": "Gel photographs extracted from supplied screenshots by rectangular cropping only. No band, brightness, contrast or colour changes. Personal chat content and reagent recipes excluded.",
  "annotation_handling": "Lane maps transcribed from source annotations. Proprietary lysis approaches are represented as A/B; SC and NC abbreviations are not expanded without confirmation. Counts, times and sample identities are source annotations, not independently verified measurements.",
  "records": [
    {
      "id": "blood-pcr",
      "title": "Bacterial preparation in a blood matrix",
      "short": "Blood + PCR",
      "method": "16S PCR",
      "organism": "Klebsiella; a second organism labelled “S. Auris”",
      "source": "1000314385.png",
      "crop": [
        31,
        425,
        486,
        316
      ],
      "image": "assets/experiments/blood-matrix-pcr.png",
      "question": "Can prepared bacterial material from a blood matrix enter a conventional PCR workflow?",
      "design": "The source describes cultured-cell pellets suspended in whole blood, with three sampled input volumes for each organism and a PBS comparison lane.",
      "observation": "Several sample lanes show bands at a similar migration position. The source-labelled PBS comparison and SC lanes also show signal.",
      "supports": "A qualitative feasibility example for sample preparation followed by bacterial 16S PCR in this experiment.",
      "limits": "The source label “S. Auris” needs identity confirmation. The blood matrix is experimentally prepared, not a clinical diagnostic cohort. No NC lane is identified in this record; band identity, expected amplicon size and specificity remain unverified.",
      "next": "Confirm the organism label, define the controls and amplicon size, and repeat matched blood-matrix and reference-method comparisons.",
      "lanes": [
        [
          "1",
          "1 kb DNA ladder"
        ],
        [
          "2–4",
          "“S. Auris”: 5, 10 and 50 µL sampled from the suspension; organism identity to confirm"
        ],
        [
          "5–7",
          "Klebsiella: 5, 10 and 50 µL sampled from the suspension"
        ],
        [
          "8",
          "Swift preparation / Klebsiella in PBS, as annotated"
        ],
        [
          "9",
          "SC, as annotated; abbreviation not defined"
        ]
      ],
      "date": "Not supplied",
      "image_width": 486,
      "image_height": 316,
      "source_sha256": "4ed732b19f78cda83cda117ea2193eb1cbee4bca836197d52f031e7a2aa46ce2",
      "figure_sha256": "327e060d1f3061a76d4004997d58f66be0377a6d1f2094220352c283294b2c30"
    },
    {
      "id": "acinetobacter-pcr",
      "title": "Acinetobacter preparation-variable study",
      "short": "Acinetobacter",
      "method": "16S PCR",
      "organism": "Acinetobacter baumannii",
      "source": "1000314387.png",
      "crop": [
        31,
        350,
        436,
        475
      ],
      "image": "assets/experiments/acinetobacter-pcr.png",
      "question": "How does preparation condition affect downstream bacterial PCR?",
      "design": "The source compares a higher cell input with three preparation-time conditions at another annotated input. SC and NC positions are recorded.",
      "observation": "Band visibility differs across the sample lanes in this photographed gel. That variation is a development finding to investigate.",
      "supports": "Documented exploration of preparation variables and downstream PCR compatibility for A. baumannii.",
      "limits": "The source-annotated cell counts are not independently verified and their input basis is unspecified. One photographed run cannot establish an optimum, detection limit or reproducibility. Expected product size and the SC/NC definitions require confirmation.",
      "next": "Repeat the controlled comparison with fixed input, confirmed control identities and pre-defined interpretation criteria.",
      "lanes": [
        [
          "1",
          "1 kb DNA ladder"
        ],
        [
          "2",
          "9 × 10⁸ cells, as annotated"
        ],
        [
          "3",
          "1 × 10⁷ cells; 10-minute preparation condition"
        ],
        [
          "4",
          "1 × 10⁷ cells; 20-minute preparation condition"
        ],
        [
          "5",
          "1 × 10⁷ cells; 30-minute preparation condition"
        ],
        [
          "6",
          "SC, as annotated; definition pending"
        ],
        [
          "7",
          "NC, as annotated; definition pending"
        ]
      ],
      "date": "Not supplied",
      "image_width": 436,
      "image_height": 475,
      "source_sha256": "c9b41814b1112f6b3ded3e377cf9bd504cd89f9510559504548fc93f674a9980",
      "figure_sha256": "1ca1d723d21a387c0fe2eb70d3ec46dfdea8f1cff752c49a2f60d43b4d86c05b"
    },
    {
      "id": "candida-comparison",
      "title": "Swift preparation beside a spin-column comparator",
      "short": "PCR comparator",
      "method": "Conventional PCR",
      "organism": "Candida albicans / Candida glabrata",
      "source": "1000314393.png",
      "crop": [
        31,
        596,
        437,
        309
      ],
      "image": "assets/experiments/candida-pcr-comparison.png",
      "question": "Can Swift-prepared material support PCR alongside a conventional extraction comparator?",
      "design": "The source identifies C. albicans-specific primers, Swift and spin-column C. albicans preparations, a spin-column C. glabrata lane and an NC lane.",
      "observation": "Bands are visible in the lanes annotated as Swift-prepared and spin-column-prepared C. albicans. The non-target and NC lanes require review at the expected product size.",
      "supports": "A side-by-side qualitative PCR compatibility example, with a named extraction comparator in the same record.",
      "limits": "This is not a quantitative equivalence, extraction-yield or assay-specificity study. A faint signal cannot be assigned to a target without its expected size and run interpretation. Matched inputs, independent samples, repeats and NC definition are unconfirmed.",
      "next": "Document matched inputs and control identities, then compare repeat runs using a defined amplicon size and acceptance criteria.",
      "lanes": [
        [
          "1",
          "100 bp DNA ladder"
        ],
        [
          "2",
          "Swift-prepared C. albicans"
        ],
        [
          "3",
          "Spin-column-prepared C. albicans"
        ],
        [
          "4",
          "Spin-column-prepared C. glabrata"
        ],
        [
          "5",
          "NC, as annotated; definition pending"
        ]
      ],
      "date": "Not supplied",
      "image_width": 437,
      "image_height": 309,
      "source_sha256": "62ec34527228d1bf079106d17cb50544c107e8a86141ded92963e3b610faf873",
      "figure_sha256": "bcf05216b7227df6606fb464a11138567aa67a6f0a3749ef25982902f7138a37"
    },
    {
      "id": "leptospira-lamp",
      "title": "Leptospira sample-preparation-to-LAMP work",
      "short": "Leptospira LAMP",
      "method": "LAMP / gel readout",
      "organism": "Leptospira, species not specified",
      "source": "1000314390.png",
      "crop": [
        31,
        480,
        437,
        418
      ],
      "image": "assets/experiments/leptospira-lamp.png",
      "question": "Can different preparation routes feed the developing LAMP workflow?",
      "design": "The source labels culture preparations in a centrifuge tube and Swift device, using two lysis approaches, plus a blood sample labelled positive. The gel is annotated as 2%.",
      "observation": "The photographed gel shows multi-band/smeared amplification patterns in several lanes. Such patterns alone do not establish target-specific amplification.",
      "supports": "An internal record linking preparation-route development with downstream LAMP experimentation.",
      "limits": "No negative-control lane is listed. The label “positive blood sample” does not establish a clinical reference result or patient cohort. Species, target, control evidence and specificity are unconfirmed. This does not verify the SensoAid hardware.",
      "next": "Add and define negative/extraction controls, verify target identity and test the complete workflow against a suitable reference.",
      "lanes": [
        [
          "1",
          "100 bp DNA ladder"
        ],
        [
          "2",
          "Centrifuge-tube culture preparation, approach A"
        ],
        [
          "3",
          "Centrifuge-tube culture preparation, approach B"
        ],
        [
          "4",
          "Swift-device culture preparation, approach A"
        ],
        [
          "5",
          "Swift-device culture preparation, approach B"
        ],
        [
          "6",
          "Swift-device blood sample labelled positive"
        ]
      ],
      "date": "Not supplied",
      "image_width": 437,
      "image_height": 418,
      "source_sha256": "6d241bb69509762f5ff7c928d2c4b81b6dc681b0c16cf727f7e6db76d5b10152",
      "figure_sha256": "de4aefa7acccd547e924db4bf785e85489c30b7f34deec8cc26d256fa81fd6ff"
    },
    {
      "id": "candida-lamp",
      "title": "Swift-prepared Candida enters LAMP",
      "short": "Candida LAMP",
      "method": "LAMP / gel readout",
      "organism": "Candida albicans",
      "source": "1000314401.png",
      "crop": [
        31,
        378,
        437,
        525
      ],
      "image": "assets/experiments/candida-lamp.png",
      "question": "Can Swift-prepared C. albicans material enter the experimental LAMP pathway?",
      "design": "The source identifies C. albicans-specific LAMP primers and compares Swift-prepared material with an SC-labelled sample.",
      "observation": "Both annotated sample lanes show prominent multi-band/smeared amplification patterns.",
      "supports": "A qualitative preparation-to-amplification development example using the Swift workflow.",
      "limits": "No negative-control lane is listed. The cell input is source-annotated, not independently quantified. A LAMP-like pattern does not establish target specificity, clinical accuracy or final-device performance; SC is not defined.",
      "next": "Confirm the SC identity and input basis; add defined negative controls and verify target-specific amplification across repeat runs.",
      "lanes": [
        [
          "1",
          "100 bp DNA ladder"
        ],
        [
          "2",
          "SwiftDNA-prepared C. albicans; 1.3 × 10⁸ cells, as annotated"
        ],
        [
          "3",
          "SC C. albicans, as annotated; SC definition pending"
        ]
      ],
      "date": "Not supplied",
      "image_width": 437,
      "image_height": 525,
      "source_sha256": "e9fb56cf9afbd8f696d311ad71684cfa8ca844a573b30d2f0b30f8ab8ba82acd",
      "figure_sha256": "420d3683211b768144a4ade0a08c4208ac097d2e77fbfc3cd2c8c67a5f49397b"
    }
  ]
}